PIPES

From Wikipedia, the free encyclopedia

Script error: No such module "about". Page Template:Chembox/styles.css has no content.

Template:Chembox IndexlistTemplate:Chembox CompToxTemplate:Chembox OHS (set)Template:Chembox Datapage check
PIPES
Lua error in package.lua at line 80: module 'Module:InfoboxImage/data' not found.
Names
Preferred IUPAC name
2,2Template:Prime-(Piperazine-1,4-diyl)di(ethane-1-sulfonic acid)
Other names
PIPES
Identifiers
Page Template:Plainlist/styles.css has no content.
3D model (JSmol)
 Page Template:Plainlist/styles.css has no content.
ChemSpider Page Template:Plainlist/styles.css has no content.
EC Number Page Template:Plainlist/styles.css has no content.
Page Template:Plainlist/styles.css has no content.
RTECS number Page Template:Plainlist/styles.css has no content.
UNII Page Template:Plainlist/styles.css has no content.
  • InChI=1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16) ☒N
    Key: IHPYMWDTONKSCO-UHFFFAOYSA-N ☒N
  • InChI=1/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)
    Key: IHPYMWDTONKSCO-UHFFFAOYAG
  • C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
Properties
C8H18N2O6S2
Molar mass 302.37
Appearance White powder
Melting point Decomposes above 300 °C
Boiling point Decomposes
1 g/L (100 °C)
Hazards
NFPA 704 (fire diamond) Page Template:NFPA 704 diamond/styles.css has no content.
NFPA 704 four-colored diamondHealth 1: Exposure would cause irritation but only minor residual injury. E.g. turpentineFlammability 0: Will not burn. E.g. waterInstability 0: Normally stable, even under fire exposure conditions, and is not reactive with water. E.g. liquid nitrogenSpecial hazards (white): no code
1
0
0
Safety data sheet (SDS) External MSDS
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
☒N verify (what is checkY☒N ?)

Template:Chembox Footer/trackingTemplate:Short description

PIPES (piperazine-N,NTemplate:Prime-bis(2-ethanesulfonic acid)) is a frequently used buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s.[1]

Applications

PIPES has two pKa values. One pKa (6.76 at 25 °C) is near the physiological pH which makes it useful in cell culture work. Its effective buffering range is 6.1-7.5 at 25 °C. The second pKa value is at 2.67 with a buffer range of from 1.5-3.5. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues.[2][3] Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer.[4] It has a negligible capacity to bind divalent ions.[5]

See also

References

Page Template:Reflist/styles.css has no content.

  1. ^ Page Module:Citation/CS1/styles.css has no content.Good, Norman E.; Winget, G. Douglas; Winter, Wilhelmina; Connolly, Thomas N.; Izawa, Seikichi; Singh, Raizada M. M. (1966). "Hydrogen Ion Buffers for Biological Research". Biochemistry. 5 (2): 467–77. doi:10.1021/bi00866a011. PMID 5942950.
  2. ^ Salema, R. and Brando, I., J. Submicr. Cytol., 9, 79 (1973).
  3. ^ Schiff, R.I. and Gennaro, J.F., Scaning Electron Microsc., 3, 449 (1979).
  4. ^ Page Module:Citation/CS1/styles.css has no content.Hardham, A.R. (1985). "Studies on the cell surface of zoospores and cysts of the fungus Phytophthora cinnamomi: The influence of fixation on patterns of lectin binding". Journal of Histochemistry. 33 (2): 110–8. doi:10.1177/33.2.3918095. PMID 3918095.
  5. ^ Page Module:Citation/CS1/styles.css has no content."Hopax Fine Chemicals - Biological buffers and their interactions with metal ions".

Template:Piperazines


Template:Asbox

Retrieved from "https://cosmopedia.co/index.php?title=PIPES&oldid=2073229"