CAPS (buffer)
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| Names | |
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| Preferred IUPAC name
3-(Cyclohexylamino)propane-1-sulfonic acid | |
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3D model (JSmol)
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PubChem CID
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| Properties | |
| C9H19NO3S | |
| Molar mass | 221.32 g/mol |
| Melting point | >300 °C |
| Acidity (pKa) | 10.4[2] |
| Hazards | |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Template:Chembox Footer/trackingTemplate:Short description
CAPS is the common name for 3-(Cyclohexylamino)-1-propanesulfonic acid, a compound used as a buffering agent in biochemistry. The similar substance N-cyclohexyl-2-hydroxyl-3-aminopropanesulfonic acid (CAPSO) is also used as buffering agent in biochemistry. Its useful pH range is 9.7-11.1. CAPS is a Good's buffer that is chemically stable and is commonly used in biochemical and analytical applications such as capillary electrophoresis.[3]
Chemical Properties
CAPS is one of Good's buffers, a class of zwitterionic buffers widely used in biochemical research. These buffers are designed to be chemically inert, non-toxic, weakly chelating, and relatively stable across temperature changes. CAPS is highly soluble in water but exhibits low solubility in organic solvents, due to its high polarity.[4]
Chemical Reactivity and Applications
Compared to several other Good's buffers, CAPS shows different behavior in metal-catalyzed oxidation systems. Many Good's buffers will readily form nitrogen-centered free radicals in the presence of Au(III) at neutral pH, leading to the cleavage of DNA. CAPS, under the same conditions, does not produce detectable free radicals or induce DNA cleavage.[5]
CAPS is used in capillary electrophoresis for the determination of pKa values for compounds that ionize at high pH. This includes weakly basic and weakly acidic compounds, such as amines and phenols. Its effectiveness arises from its minimal interaction with analytes reducing interference and allowing measurements to more accurately reflect the analyte.[3]
See also
References
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- ^ CAPS at Sigma-Aldrich
- ^ Page Module:Citation/CS1/styles.css has no content.Esplin, Taran L.; Cable, Morgan L.; Gray, Harry B.; Ponce, Adrian (2010). "Terbium-Macrocycle Complexes as Chemical Sensors: Detection of an Aspirin Metabolite in Urine Using a Salicylurate-Specific Receptor Site". Inorganic Chemistry. 49 (10): 4643–4647. doi:10.1021/ic1003066.
Page Module:Citation/CS1/styles.css has no content.Tabata, Masaaki; Habib, Ahsan; Watanabe, Keiichi (2005). "DNA Cleavage by Good's Buffers in the Presence of Au(III)". Bulletin of the Chemical Society of Japan. 78 (7): 1263–1269. doi:10.1246/bcsj.78.1263. - ^ a b Page Module:Citation/CS1/styles.css has no content.Fuguet, Elisabet; Reta, Mario; Gibert, Carme; Rosés, Martí; Bosch, Elisabeth; Ràfols, Clara (July 8, 2008). "Critical evaluation of buffering solutions for p K a determination by capillary electrophoresis". ELECTROPHORESIS. 29 (13): 2841–2851. doi:10.1002/elps.200700869. ISSN 0173-0835.
- ^ Page Module:Citation/CS1/styles.css has no content.Taha, Mohamed; Coutinho, João A.P. (June 5, 2016). "Organic-phase biological buffers for biochemical and biological research in organic media". Journal of Molecular Liquids. 221: 197–205. doi:10.1016/j.molliq.2016.05.052.
- ^ Page Module:Citation/CS1/styles.css has no content.Tabata, Masaaki; Habib, Ahsan; Watanabe, Keiichi (2005-07-01). "DNA Cleavage by Good's Buffers in the Presence of Au(III)". Bulletin of the Chemical Society of Japan. 78 (7): 1263–1269. doi:10.1246/bcsj.78.1263. ISSN 0009-2673.
